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superscript ii reverse transcriptase  (TaKaRa)


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    Structured Review

    TaKaRa superscript ii reverse transcriptase
    Superscript Ii Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 736 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+reverse+transcriptase/PrimeScript+II+Reverse+Transcriptase/pmc12664801-200-12-16
    Average 96 stars, based on 736 article reviews
    superscript ii reverse transcriptase - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Synthesized:

    Article Title: Water extract from artichoke ameliorates high-fat diet-induced non-alcoholic fatty liver disease in rats
    Article Snippet: .. Briefly, cDNA was synthesized from 1 μg of RNA using a primer mix (oligo-dT/random primers) and Superscript reverse transcriptase (Takara, Dalian, China), according to the manufacturer’s instructions. .. Real-time PCR was performed in triplicate for each sample, using a 20 μL reaction volume and a SYBR Green universal PCR mix (Takara).

    Article Title: Water extract from artichoke ameliorates high-fat diet-induced non-alcoholic fatty liver disease in rats.
    Article Snippet: .. Briefly, cDNA was synthesized from 1 μg of RNA using a primer mix (oligo-dT/random primers) and Superscript reverse transcriptase (Takara, Dalian, China), according to the manufacturer’s instructions. .. Real-time PCR was performed in triplicate for each sample, using a 20 μL reaction volume and a SYBR Green universal PCR mix (Takara).

    Reverse Transcription:

    Article Title: Water extract from artichoke ameliorates high-fat diet-induced non-alcoholic fatty liver disease in rats
    Article Snippet: .. Briefly, cDNA was synthesized from 1 μg of RNA using a primer mix (oligo-dT/random primers) and Superscript reverse transcriptase (Takara, Dalian, China), according to the manufacturer’s instructions. .. Real-time PCR was performed in triplicate for each sample, using a 20 μL reaction volume and a SYBR Green universal PCR mix (Takara).

    Article Title: Water extract from artichoke ameliorates high-fat diet-induced non-alcoholic fatty liver disease in rats.
    Article Snippet: .. Briefly, cDNA was synthesized from 1 μg of RNA using a primer mix (oligo-dT/random primers) and Superscript reverse transcriptase (Takara, Dalian, China), according to the manufacturer’s instructions. .. Real-time PCR was performed in triplicate for each sample, using a 20 μL reaction volume and a SYBR Green universal PCR mix (Takara).

    Article Title: Enterococcus faecium HDRsEf1 Represses CYP3A29 Expression in the Intestine through the TLR1/2-induced A20 to attenuate the NF-κB/RXR-α Signaling
    Article Snippet: .. Quantitative Real-Time PCR (qRT-PCR) The relative levels of target gene to the control β-actin mRNA transcripts were determined by qRT-PCR using speci c primers (Table S1) and iQTM SYBR Green PCR Supermix in the Bio-Rad CXF real-time PCR detection system (Bio-Rad, CA, USA) after we extracted total RNA from individual groups of cells with the TRIzol reagent (Invitrogen) and reversely transcribed (RT) into cDNA using the Superscript reverse transcriptase (Takara, Otsu, Japan). ..

    Article Title: A Cocktail of Three Virulent Phages Controls Multidrug-Resistant Salmonella Enteritidis Infection in Poultry
    Article Snippet: Small intestine samples were collected and total RNA was isolated (100 mg) using Trizol reagent (Invitrogen, Carlsbad, CA, United States) according to manufacturer’s instruction. .. Extracted RNA was quantified to 1 μg/μL and reverse transcription was performed with the Superscript reverse transcriptase (Takara, Otsu, Shiga, Japan). .. Cytokine gene expression levels were detected in the Bio-Rad CXF real-time PCR (Bio-Rad, Hercules, CA, United States) using iQTM SYBR Green PCR Supermix (Takara, Otsu, Japan).

    Article Title: Genome- and transcriptome-wide identification of trehalose-6-phosphate phosphatases ( TPP ) gene family and their expression patterns under abiotic stress and exogenous trehalose in soybean
    Article Snippet: .. Moreover, the total RNA from the T + SA treatment was reverse transcribed using oligo (dT) primer and SuperScript Reverse Transcriptase (TaKaRa, Nanjing, China). qRT-PCR was then performed using SYBR green (TaKaRa Biotechnology) on Roche Cycler 480II system (Roche, Roche Diagnostics, Switzerland). ..

    Article Title: A Cocktail of Three Virulent Phages Controls Multidrug-Resistant Salmonella Enteritidis Infection in Poultry.
    Article Snippet: –, no phage treatment; –1, phage cocktail administered 1 day before Salmonella infection. using Trizol reagent (Invitrogen, Carlsbad, CA, United States) according to manufacturer’s instruction. .. Extracted RNA was quantified to 1 μg/μL and reverse transcription was performed with the Superscript reverse transcriptase (Takara, Otsu, Shiga, Japan). .. Cytokine gene expression levels were detected in the Bio-Rad CXF real-time PCR (Bio-Rad, Hercules, CA, United States) using iQTM SYBR Green PCR Supermix (Takara, Otsu, Japan).

    Article Title: H3K36 demethylase JMJ710 negatively regulates drought tolerance by suppressing MYB48-1 expression in rice.
    Article Snippet: RNase-free DNase I (Thermo Fisher Scientific, Waltham, MA, USA; EN0521) was used to remove genomic DNA. .. The RNA was reverse transcribed using a SuperScript reverse transcriptase (TaKaRA; RR047A) based on the manufacturer’s instructions. .. The qPCR was performed with SYBR Premix ExTaq II (TaKaRa; RR820A) using a Biorad CFX96 touch real-time PCR system (Applied Biosystems, Waltham, MA, USA).

    Article Title: Expression Patterns of Ezrin and AJAP1 and Clinical Significance in Breast Cancer
    Article Snippet: Total RNA was isolated from cell lines using TRIzol reagent (Invitrogen, Inc.) under the manufacturer’s protocols. .. Then the RNA was reversed transcribed to cDNA using SuperScript Reverse Transcriptase (Takara, Shiga, Japan). .. Reactions were performed using the SYBR Green PCR Kit (Takara, Japan).

    Real-time Polymerase Chain Reaction:

    Article Title: Enterococcus faecium HDRsEf1 Represses CYP3A29 Expression in the Intestine through the TLR1/2-induced A20 to attenuate the NF-κB/RXR-α Signaling
    Article Snippet: .. Quantitative Real-Time PCR (qRT-PCR) The relative levels of target gene to the control β-actin mRNA transcripts were determined by qRT-PCR using speci c primers (Table S1) and iQTM SYBR Green PCR Supermix in the Bio-Rad CXF real-time PCR detection system (Bio-Rad, CA, USA) after we extracted total RNA from individual groups of cells with the TRIzol reagent (Invitrogen) and reversely transcribed (RT) into cDNA using the Superscript reverse transcriptase (Takara, Otsu, Japan). ..

    Control:

    Article Title: Enterococcus faecium HDRsEf1 Represses CYP3A29 Expression in the Intestine through the TLR1/2-induced A20 to attenuate the NF-κB/RXR-α Signaling
    Article Snippet: .. Quantitative Real-Time PCR (qRT-PCR) The relative levels of target gene to the control β-actin mRNA transcripts were determined by qRT-PCR using speci c primers (Table S1) and iQTM SYBR Green PCR Supermix in the Bio-Rad CXF real-time PCR detection system (Bio-Rad, CA, USA) after we extracted total RNA from individual groups of cells with the TRIzol reagent (Invitrogen) and reversely transcribed (RT) into cDNA using the Superscript reverse transcriptase (Takara, Otsu, Japan). ..

    Quantitative RT-PCR:

    Article Title: Enterococcus faecium HDRsEf1 Represses CYP3A29 Expression in the Intestine through the TLR1/2-induced A20 to attenuate the NF-κB/RXR-α Signaling
    Article Snippet: .. Quantitative Real-Time PCR (qRT-PCR) The relative levels of target gene to the control β-actin mRNA transcripts were determined by qRT-PCR using speci c primers (Table S1) and iQTM SYBR Green PCR Supermix in the Bio-Rad CXF real-time PCR detection system (Bio-Rad, CA, USA) after we extracted total RNA from individual groups of cells with the TRIzol reagent (Invitrogen) and reversely transcribed (RT) into cDNA using the Superscript reverse transcriptase (Takara, Otsu, Japan). ..

    Article Title: Genome- and transcriptome-wide identification of trehalose-6-phosphate phosphatases ( TPP ) gene family and their expression patterns under abiotic stress and exogenous trehalose in soybean
    Article Snippet: .. Moreover, the total RNA from the T + SA treatment was reverse transcribed using oligo (dT) primer and SuperScript Reverse Transcriptase (TaKaRa, Nanjing, China). qRT-PCR was then performed using SYBR green (TaKaRa Biotechnology) on Roche Cycler 480II system (Roche, Roche Diagnostics, Switzerland). ..

    SYBR Green Assay:

    Article Title: Enterococcus faecium HDRsEf1 Represses CYP3A29 Expression in the Intestine through the TLR1/2-induced A20 to attenuate the NF-κB/RXR-α Signaling
    Article Snippet: .. Quantitative Real-Time PCR (qRT-PCR) The relative levels of target gene to the control β-actin mRNA transcripts were determined by qRT-PCR using speci c primers (Table S1) and iQTM SYBR Green PCR Supermix in the Bio-Rad CXF real-time PCR detection system (Bio-Rad, CA, USA) after we extracted total RNA from individual groups of cells with the TRIzol reagent (Invitrogen) and reversely transcribed (RT) into cDNA using the Superscript reverse transcriptase (Takara, Otsu, Japan). ..

    Article Title: Genome- and transcriptome-wide identification of trehalose-6-phosphate phosphatases ( TPP ) gene family and their expression patterns under abiotic stress and exogenous trehalose in soybean
    Article Snippet: .. Moreover, the total RNA from the T + SA treatment was reverse transcribed using oligo (dT) primer and SuperScript Reverse Transcriptase (TaKaRa, Nanjing, China). qRT-PCR was then performed using SYBR green (TaKaRa Biotechnology) on Roche Cycler 480II system (Roche, Roche Diagnostics, Switzerland). ..

    Polymerase Chain Reaction:

    Article Title: Enterococcus faecium HDRsEf1 Represses CYP3A29 Expression in the Intestine through the TLR1/2-induced A20 to attenuate the NF-κB/RXR-α Signaling
    Article Snippet: .. Quantitative Real-Time PCR (qRT-PCR) The relative levels of target gene to the control β-actin mRNA transcripts were determined by qRT-PCR using speci c primers (Table S1) and iQTM SYBR Green PCR Supermix in the Bio-Rad CXF real-time PCR detection system (Bio-Rad, CA, USA) after we extracted total RNA from individual groups of cells with the TRIzol reagent (Invitrogen) and reversely transcribed (RT) into cDNA using the Superscript reverse transcriptase (Takara, Otsu, Japan). ..



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